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trail neutralizing antibody  (R&D Systems)


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    R&D Systems trail neutralizing antibody
    Trail Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+trail+tnfsf10/Human+TRAIL%2FTNFSF10+Antibody/pmc13111737-305-11-15
    Average 94 stars, based on 27 article reviews
    trail neutralizing antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced mitochondrial pathway to apoptosis and caspase activation is potentiated by phospholipid scramblase-3
    Article Snippet: .. Briefly, after treatment with 10 ng/ml of recombinant human TRAIL/TNFSF10 (R&D systems, Minneapolis, MN), 10 6 cells were collected by centrifugation, washed and then resuspended in PBS containing 200 nM NAO (Molecular Probes, Eugene, OR) for 30 min at room temperature. ..

    Article Title: The fibronectin III-1 domain activates a PI3-Kinase/Akt signaling pathway leading to αvβ5 integrin activation and TRAIL resistance in human lung cancer cells
    Article Snippet: .. Cells were stimulated with recombinant human TRAIL/TNFSF10 (TRAIL) (R&D systems) or PBS as a control treatment as indicated in the figure legends. ..

    Article Title: Irradiation specifically sensitises solid tumour cell lines to TRAIL mediated apoptosis
    Article Snippet: .. TRAIL induced apoptosis was induced with recombinant human TRAIL/TNFSF10 (R&D Systems, Wiesbaden-Nordenstadt, Germany) in concomitant or sequential application with irradiation. .. Cells were irradiated with 6 MV Photons using a Siemens Mevatron linear accelerator with a dose rate of 4 Gy per min at room temperature.

    Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines
    Article Snippet: .. This procedure was performed according to the manufacturer’s recommendations for human TRAIL/TNFSF10 (DY375, R&D) but using anti-human TRAIL (MAB3751, R&D) as capture Ab; recombinant human TRAIL (rhTRAIL, 375-TL, R&D, at 31.25 to 2,000 pg/ml) to produce a standard curve; and anti-human TRAIL (BAF375, R&D) as a detection Ab together with streptavidin-peroxidase (S5512, Sigma). .. The enzyme activity was revealed using a 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Sigma), and it was abolished using 1 N HCl (Merck).

    Centrifugation:

    Article Title: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced mitochondrial pathway to apoptosis and caspase activation is potentiated by phospholipid scramblase-3
    Article Snippet: .. Briefly, after treatment with 10 ng/ml of recombinant human TRAIL/TNFSF10 (R&D systems, Minneapolis, MN), 10 6 cells were collected by centrifugation, washed and then resuspended in PBS containing 200 nM NAO (Molecular Probes, Eugene, OR) for 30 min at room temperature. ..

    Control:

    Article Title: The fibronectin III-1 domain activates a PI3-Kinase/Akt signaling pathway leading to αvβ5 integrin activation and TRAIL resistance in human lung cancer cells
    Article Snippet: .. Cells were stimulated with recombinant human TRAIL/TNFSF10 (TRAIL) (R&D systems) or PBS as a control treatment as indicated in the figure legends. ..

    Irradiation:

    Article Title: Irradiation specifically sensitises solid tumour cell lines to TRAIL mediated apoptosis
    Article Snippet: .. TRAIL induced apoptosis was induced with recombinant human TRAIL/TNFSF10 (R&D Systems, Wiesbaden-Nordenstadt, Germany) in concomitant or sequential application with irradiation. .. Cells were irradiated with 6 MV Photons using a Siemens Mevatron linear accelerator with a dose rate of 4 Gy per min at room temperature.

    Enzyme-linked Immunosorbent Assay:

    Article Title: High Serum IgG4 Concentrations in Patients with Hashimoto's Thyroiditis
    Article Snippet: IgG4 was estimated using Human IgG4 Platinum ELISA (eBioscience, Vienna, Austria). .. The following chemokines and apoptotic markers were also measured: Human Fas Ligand/TNFSF6, Human TRAIL/TNFSF10, Human TGF- β 1, and chemokines CXCL9/I-TAC, CXCL10/IP-10, and CXCL11/I-TAC (Human Quantikine ELISA R&D Systems, Minneapolis, USA). .. STATISTICA 10.0 for Windows (StatSoft.



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    (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and <t>TRAIL</t> secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.
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    (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and TRAIL secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and TRAIL secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Luciferase, Cytotoxicity Assay, Transfection, Cell Culture, Control, Luminex, Two Tailed Test

    (A) FACS analysis to determine the surface expression of IFNG-R1, TNF-R1, TNF-R2, FAS, TRAIL-R1, and TRAIL-R2 in MO3.13-A2-Luc cells. Grey histograms represent the isotype control. (B) Impact of IRG knockdown on TRAIL-R2 surface expression in MO3.13-A2-Luc cells. Left panel: Representative overlay of histograms to compare TRAIL-R2 expression on IRG +/- MO3.13-A2-Luc cells. Right panel: Compiled data of mean fluorescence intensity (MFI) for TRAIL-R2 expression. (C) Real-time cytotoxicity assay (IncuCyte® SX5 System) to analyze TRAIL-induced apoptosis over 48 hours in IRG +/- MO3.13-A2-Luc cells. Incucyte® Cytotox-Red Dye was added to the transfected MO3.13 oligodendrocytes together with TRAIL treatment as an indicator of apoptosis. The graph shows total red object integrated intensity per well (RCU x µm²/Image). (D) Western blot analysis of total/cleaved caspase-3/8/9, TRAF2, phosho(p)/total TAK1, MKK4, JNK, NF-κB, pLKB1 and Bcl2 in IRG +/- MO3.13-A2-Luc cells upon 4 h treatment with TRAIL. Experiment was run in two separate blots each including Scr samples. Each HIT was shown in comparison to Scr sample analyzed in the same blot (Blot1: Scr, siSTK11, siKCNH8 and siABCA2; Blot2: Scr, siSLC1A3 and siCHRNA1). Representative data of (A & D) two, (B-C) three independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001).

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A) FACS analysis to determine the surface expression of IFNG-R1, TNF-R1, TNF-R2, FAS, TRAIL-R1, and TRAIL-R2 in MO3.13-A2-Luc cells. Grey histograms represent the isotype control. (B) Impact of IRG knockdown on TRAIL-R2 surface expression in MO3.13-A2-Luc cells. Left panel: Representative overlay of histograms to compare TRAIL-R2 expression on IRG +/- MO3.13-A2-Luc cells. Right panel: Compiled data of mean fluorescence intensity (MFI) for TRAIL-R2 expression. (C) Real-time cytotoxicity assay (IncuCyte® SX5 System) to analyze TRAIL-induced apoptosis over 48 hours in IRG +/- MO3.13-A2-Luc cells. Incucyte® Cytotox-Red Dye was added to the transfected MO3.13 oligodendrocytes together with TRAIL treatment as an indicator of apoptosis. The graph shows total red object integrated intensity per well (RCU x µm²/Image). (D) Western blot analysis of total/cleaved caspase-3/8/9, TRAF2, phosho(p)/total TAK1, MKK4, JNK, NF-κB, pLKB1 and Bcl2 in IRG +/- MO3.13-A2-Luc cells upon 4 h treatment with TRAIL. Experiment was run in two separate blots each including Scr samples. Each HIT was shown in comparison to Scr sample analyzed in the same blot (Blot1: Scr, siSTK11, siKCNH8 and siABCA2; Blot2: Scr, siSLC1A3 and siCHRNA1). Representative data of (A & D) two, (B-C) three independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001).

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Expressing, Control, Knockdown, Fluorescence, Cytotoxicity Assay, Transfection, Western Blot, Comparison, Two Tailed Test

    (A-B) Impact of SIK3 on TRAIL-mediated MO3.13-A2-Luc killing was determined by (A) Luciferase-based cytotoxicity assay as described in supp and (B) real-time cytotoxicity assay as described in . Representative data of (A) three (B) two independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01).

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A-B) Impact of SIK3 on TRAIL-mediated MO3.13-A2-Luc killing was determined by (A) Luciferase-based cytotoxicity assay as described in supp and (B) real-time cytotoxicity assay as described in . Representative data of (A) three (B) two independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01).

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Luciferase, Cytotoxicity Assay, Two Tailed Test

    Summary of molecular pathways involved in the downstream signaling of STK11/LKB1, KCNH8, ABCA2, SLC1A3/EAAT1 and/or CHRNA1/AChR-mediated immune resistance. Activation of caspase-3/-8/-9, MKK4, JNK1, NF-κB and expression of TRAF2, TRAIL-R2, IL-8 was experimentally depicted in this study, whereas the potential involvement of the other molecular components was deducted from the previous studies as described and referred in the results and discussion sections above. Colors of the lines depicting the regulatory networks are matched with colors of the IRG icons. Activating and inhibitory effects are indicated by arrows and blocked lines respectively. The figure was generated using biorender.com (Created in BioRender. Beckhove, P. (2026) https://BioRender.com/2adzain )

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: Summary of molecular pathways involved in the downstream signaling of STK11/LKB1, KCNH8, ABCA2, SLC1A3/EAAT1 and/or CHRNA1/AChR-mediated immune resistance. Activation of caspase-3/-8/-9, MKK4, JNK1, NF-κB and expression of TRAF2, TRAIL-R2, IL-8 was experimentally depicted in this study, whereas the potential involvement of the other molecular components was deducted from the previous studies as described and referred in the results and discussion sections above. Colors of the lines depicting the regulatory networks are matched with colors of the IRG icons. Activating and inhibitory effects are indicated by arrows and blocked lines respectively. The figure was generated using biorender.com (Created in BioRender. Beckhove, P. (2026) https://BioRender.com/2adzain )

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Activation Assay, Expressing, Generated